Gene probing reveals the widespread distribution, diversity and abundance of isoprene-degrading bacteria in the environment.
Identifieur interne : 000E80 ( Main/Exploration ); précédent : 000E79; suivant : 000E81Gene probing reveals the widespread distribution, diversity and abundance of isoprene-degrading bacteria in the environment.
Auteurs : Ornella Carri N [Royaume-Uni] ; Nasmille L. Larke-Mejía [Royaume-Uni] ; Lisa Gibson [Royaume-Uni] ; Muhammad Farhan Ul Haque [Royaume-Uni] ; Javier Ramiro-García [Luxembourg (pays)] ; Terry J. Mcgenity [Royaume-Uni] ; J Colin Murrell [Royaume-Uni]Source :
- Microbiome [ 2049-2618 ] ; 2018.
Descripteurs français
- KwdFr :
- Amorces ADN (génétique), Analyse de séquence d'ADN (MeSH), Bactéries (classification), Bactéries (enzymologie), Bactéries (isolement et purification), Butadiènes (métabolisme), Comamonadaceae (classification), Comamonadaceae (enzymologie), Comamonadaceae (isolement et purification), Dépollution biologique de l'environnement (MeSH), Hémiterpènes (métabolisme), Microbiologie du sol (MeSH), Mixed function oxygenases (génétique), Phylogenèse (MeSH), Protéines bactériennes (génétique), Rhodococcus (classification), Rhodococcus (enzymologie), Rhodococcus (isolement et purification), Sphingomonadaceae (classification), Sphingomonadaceae (enzymologie), Sphingomonadaceae (isolement et purification).
- MESH :
- enzymologie : Bactéries, Comamonadaceae, Rhodococcus, Sphingomonadaceae.
- génétique : Amorces ADN, Bactéries, Comamonadaceae, Mixed function oxygenases, Protéines bactériennes, Rhodococcus, Sphingomonadaceae.
- isolement et purification : Bactéries, Comamonadaceae, Rhodococcus, Sphingomonadaceae.
- métabolisme : Butadiènes, Hémiterpènes.
- Analyse de séquence d'ADN, Dépollution biologique de l'environnement, Microbiologie du sol, Phylogenèse.
English descriptors
- KwdEn :
- Bacteria (classification), Bacteria (enzymology), Bacteria (isolation & purification), Bacterial Proteins (genetics), Biodegradation, Environmental (MeSH), Butadienes (metabolism), Comamonadaceae (classification), Comamonadaceae (enzymology), Comamonadaceae (isolation & purification), DNA Primers (genetics), Hemiterpenes (metabolism), Mixed Function Oxygenases (genetics), Phylogeny (MeSH), Rhodococcus (classification), Rhodococcus (enzymology), Rhodococcus (isolation & purification), Sequence Analysis, DNA (MeSH), Soil Microbiology (MeSH), Sphingomonadaceae (classification), Sphingomonadaceae (enzymology), Sphingomonadaceae (isolation & purification).
- MESH :
- chemical , genetics : Bacterial Proteins, DNA Primers, Mixed Function Oxygenases.
- classification : Bacteria, Comamonadaceae, Rhodococcus, Sphingomonadaceae.
- enzymology : Bacteria, Comamonadaceae, Rhodococcus, Sphingomonadaceae.
- isolation & purification : Bacteria, Comamonadaceae, Rhodococcus, Sphingomonadaceae.
- chemical , metabolism : Butadienes, Hemiterpenes.
- Biodegradation, Environmental, Phylogeny, Sequence Analysis, DNA, Soil Microbiology.
Abstract
BACKGROUND
Approximately 500 Tg of isoprene are emitted to the atmosphere annually, an amount similar to that of methane, and despite its significant effects on the climate, very little is known about the biological degradation of isoprene in the environment. Isolation and characterisation of isoprene degraders at the molecular level has allowed the development of probes targeting isoA encoding the α-subunit of the isoprene monooxygenase. This enzyme belongs to the soluble diiron centre monooxygenase family and catalyses the first step in the isoprene degradation pathway. The use of probes targeting key metabolic genes is a successful approach in molecular ecology to study specific groups of bacteria in complex environments. Here, we developed and tested a novel isoA PCR primer set to study the distribution, abundance, and diversity of isoprene degraders in a wide range of environments.
RESULTS
The new isoA probes specifically amplified isoA genes from taxonomically diverse isoprene-degrading bacteria including members of the genera Rhodococcus, Variovorax, and Sphingopyxis. There was no cross-reactivity with genes encoding related oxygenases from non-isoprene degraders. Sequencing of isoA amplicons from DNA extracted from environmental samples enriched with isoprene revealed that most environments tested harboured a considerable variety of isoA sequences, with poplar leaf enrichments containing more phylogenetically diverse isoA genes. Quantification by qPCR using these isoA probes revealed that isoprene degraders are widespread in the phyllosphere, terrestrial, freshwater and marine environments. Specifically, soils in the vicinity of high isoprene-emitting trees contained the highest number of isoprene-degrading bacteria.
CONCLUSION
This study provides the molecular ecology tools to broaden our knowledge of the distribution, abundance and diversity of isoprene degraders in the environment, which is a fundamental step necessary to assess the impact that microbes have in mitigating the effects of this important climate-active gas.
DOI: 10.1186/s40168-018-0607-0
PubMed: 30526688
PubMed Central: PMC6286570
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Larke-Mejía</name><affiliation wicri:level="1"><nlm:affiliation>School of Environmental Sciences, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>School of Environmental Sciences, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ</wicri:regionArea><wicri:noRegion>NR4 7TJ</wicri:noRegion></affiliation></author><author><name sortKey="Gibson, Lisa" sort="Gibson, Lisa" uniqKey="Gibson L" first="Lisa" last="Gibson">Lisa Gibson</name><affiliation wicri:level="1"><nlm:affiliation>School of Environmental Sciences, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>School of Environmental Sciences, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ</wicri:regionArea><wicri:noRegion>NR4 7TJ</wicri:noRegion></affiliation></author><author><name sortKey="Farhan Ul Haque, Muhammad" sort="Farhan Ul Haque, Muhammad" uniqKey="Farhan Ul Haque M" first="Muhammad" last="Farhan Ul Haque">Muhammad Farhan Ul Haque</name><affiliation wicri:level="1"><nlm:affiliation>School of Environmental Sciences, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>School of Environmental Sciences, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ</wicri:regionArea><wicri:noRegion>NR4 7TJ</wicri:noRegion></affiliation></author><author><name sortKey="Ramiro Garcia, Javier" sort="Ramiro Garcia, Javier" uniqKey="Ramiro Garcia J" first="Javier" last="Ramiro-García">Javier Ramiro-García</name><affiliation wicri:level="4"><nlm:affiliation>Luxembourg Centre for Systems Biomedicine, University of Luxembourg, Esch-sur-Alzette, Luxembourg.</nlm:affiliation><country xml:lang="fr">Luxembourg (pays)</country><wicri:regionArea>Luxembourg Centre for Systems Biomedicine, University of Luxembourg, Esch-sur-Alzette</wicri:regionArea><orgName type="university">Université du Luxembourg</orgName><placeName><settlement type="city">Luxembourg</settlement></placeName></affiliation></author><author><name sortKey="Mcgenity, Terry J" sort="Mcgenity, Terry J" uniqKey="Mcgenity T" first="Terry J" last="Mcgenity">Terry J. Mcgenity</name><affiliation wicri:level="1"><nlm:affiliation>School of Biological Sciences, University of Essex, Colchester, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>School of Biological Sciences, University of Essex, Colchester</wicri:regionArea><wicri:noRegion>Colchester</wicri:noRegion></affiliation></author><author><name sortKey="Murrell, J Colin" sort="Murrell, J Colin" uniqKey="Murrell J" first="J Colin" last="Murrell">J Colin Murrell</name><affiliation wicri:level="1"><nlm:affiliation>School of Environmental Sciences, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK. j.c.murrell@uea.ac.uk.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>School of Environmental Sciences, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ</wicri:regionArea><wicri:noRegion>NR4 7TJ</wicri:noRegion></affiliation></author></analytic><series><title level="j">Microbiome</title><idno type="eISSN">2049-2618</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacteria (classification)</term><term>Bacteria (enzymology)</term><term>Bacteria (isolation & purification)</term><term>Bacterial Proteins (genetics)</term><term>Biodegradation, Environmental (MeSH)</term><term>Butadienes (metabolism)</term><term>Comamonadaceae (classification)</term><term>Comamonadaceae (enzymology)</term><term>Comamonadaceae (isolation & purification)</term><term>DNA Primers (genetics)</term><term>Hemiterpenes (metabolism)</term><term>Mixed Function Oxygenases (genetics)</term><term>Phylogeny (MeSH)</term><term>Rhodococcus (classification)</term><term>Rhodococcus (enzymology)</term><term>Rhodococcus (isolation & purification)</term><term>Sequence Analysis, DNA (MeSH)</term><term>Soil Microbiology (MeSH)</term><term>Sphingomonadaceae (classification)</term><term>Sphingomonadaceae (enzymology)</term><term>Sphingomonadaceae (isolation & purification)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Amorces ADN (génétique)</term><term>Analyse de séquence d'ADN (MeSH)</term><term>Bactéries (classification)</term><term>Bactéries (enzymologie)</term><term>Bactéries (isolement et purification)</term><term>Butadiènes (métabolisme)</term><term>Comamonadaceae (classification)</term><term>Comamonadaceae (enzymologie)</term><term>Comamonadaceae (isolement et purification)</term><term>Dépollution biologique de l'environnement (MeSH)</term><term>Hémiterpènes (métabolisme)</term><term>Microbiologie du sol (MeSH)</term><term>Mixed function oxygenases (génétique)</term><term>Phylogenèse (MeSH)</term><term>Protéines bactériennes (génétique)</term><term>Rhodococcus (classification)</term><term>Rhodococcus (enzymologie)</term><term>Rhodococcus (isolement et purification)</term><term>Sphingomonadaceae (classification)</term><term>Sphingomonadaceae (enzymologie)</term><term>Sphingomonadaceae (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Bacterial Proteins</term><term>DNA Primers</term><term>Mixed Function Oxygenases</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Bacteria</term><term>Comamonadaceae</term><term>Rhodococcus</term><term>Sphingomonadaceae</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Bactéries</term><term>Comamonadaceae</term><term>Rhodococcus</term><term>Sphingomonadaceae</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Bacteria</term><term>Comamonadaceae</term><term>Rhodococcus</term><term>Sphingomonadaceae</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Amorces ADN</term><term>Bactéries</term><term>Comamonadaceae</term><term>Mixed function oxygenases</term><term>Protéines bactériennes</term><term>Rhodococcus</term><term>Sphingomonadaceae</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Bacteria</term><term>Comamonadaceae</term><term>Rhodococcus</term><term>Sphingomonadaceae</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Bactéries</term><term>Comamonadaceae</term><term>Rhodococcus</term><term>Sphingomonadaceae</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Butadienes</term><term>Hemiterpenes</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Butadiènes</term><term>Hémiterpènes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biodegradation, Environmental</term><term>Phylogeny</term><term>Sequence Analysis, DNA</term><term>Soil Microbiology</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Dépollution biologique de l'environnement</term><term>Microbiologie du sol</term><term>Phylogenèse</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>Approximately 500 Tg of isoprene are emitted to the atmosphere annually, an amount similar to that of methane, and despite its significant effects on the climate, very little is known about the biological degradation of isoprene in the environment. Isolation and characterisation of isoprene degraders at the molecular level has allowed the development of probes targeting isoA encoding the α-subunit of the isoprene monooxygenase. This enzyme belongs to the soluble diiron centre monooxygenase family and catalyses the first step in the isoprene degradation pathway. The use of probes targeting key metabolic genes is a successful approach in molecular ecology to study specific groups of bacteria in complex environments. Here, we developed and tested a novel isoA PCR primer set to study the distribution, abundance, and diversity of isoprene degraders in a wide range of environments.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>The new isoA probes specifically amplified isoA genes from taxonomically diverse isoprene-degrading bacteria including members of the genera Rhodococcus, Variovorax, and Sphingopyxis. There was no cross-reactivity with genes encoding related oxygenases from non-isoprene degraders. Sequencing of isoA amplicons from DNA extracted from environmental samples enriched with isoprene revealed that most environments tested harboured a considerable variety of isoA sequences, with poplar leaf enrichments containing more phylogenetically diverse isoA genes. Quantification by qPCR using these isoA probes revealed that isoprene degraders are widespread in the phyllosphere, terrestrial, freshwater and marine environments. Specifically, soils in the vicinity of high isoprene-emitting trees contained the highest number of isoprene-degrading bacteria.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSION</b></p><p>This study provides the molecular ecology tools to broaden our knowledge of the distribution, abundance and diversity of isoprene degraders in the environment, which is a fundamental step necessary to assess the impact that microbes have in mitigating the effects of this important climate-active gas.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30526688</PMID><DateCompleted><Year>2019</Year><Month>04</Month><Day>03</Day></DateCompleted><DateRevised><Year>2019</Year><Month>04</Month><Day>03</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2049-2618</ISSN><JournalIssue CitedMedium="Internet"><Volume>6</Volume><Issue>1</Issue><PubDate><Year>2018</Year><Month>12</Month><Day>07</Day></PubDate></JournalIssue><Title>Microbiome</Title><ISOAbbreviation>Microbiome</ISOAbbreviation></Journal><ArticleTitle>Gene probing reveals the widespread distribution, diversity and abundance of isoprene-degrading bacteria in the environment.</ArticleTitle><Pagination><MedlinePgn>219</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/s40168-018-0607-0</ELocationID><Abstract><AbstractText Label="BACKGROUND">Approximately 500 Tg of isoprene are emitted to the atmosphere annually, an amount similar to that of methane, and despite its significant effects on the climate, very little is known about the biological degradation of isoprene in the environment. Isolation and characterisation of isoprene degraders at the molecular level has allowed the development of probes targeting isoA encoding the α-subunit of the isoprene monooxygenase. This enzyme belongs to the soluble diiron centre monooxygenase family and catalyses the first step in the isoprene degradation pathway. The use of probes targeting key metabolic genes is a successful approach in molecular ecology to study specific groups of bacteria in complex environments. Here, we developed and tested a novel isoA PCR primer set to study the distribution, abundance, and diversity of isoprene degraders in a wide range of environments.</AbstractText><AbstractText Label="RESULTS">The new isoA probes specifically amplified isoA genes from taxonomically diverse isoprene-degrading bacteria including members of the genera Rhodococcus, Variovorax, and Sphingopyxis. There was no cross-reactivity with genes encoding related oxygenases from non-isoprene degraders. Sequencing of isoA amplicons from DNA extracted from environmental samples enriched with isoprene revealed that most environments tested harboured a considerable variety of isoA sequences, with poplar leaf enrichments containing more phylogenetically diverse isoA genes. Quantification by qPCR using these isoA probes revealed that isoprene degraders are widespread in the phyllosphere, terrestrial, freshwater and marine environments. Specifically, soils in the vicinity of high isoprene-emitting trees contained the highest number of isoprene-degrading bacteria.</AbstractText><AbstractText Label="CONCLUSION">This study provides the molecular ecology tools to broaden our knowledge of the distribution, abundance and diversity of isoprene degraders in the environment, which is a fundamental step necessary to assess the impact that microbes have in mitigating the effects of this important climate-active gas.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Carrión</LastName><ForeName>Ornella</ForeName><Initials>O</Initials><Identifier Source="ORCID">0000-0002-9959-7905</Identifier><AffiliationInfo><Affiliation>School of Environmental Sciences, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK. o.carrion-fonseca@uea.ac.uk.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Larke-Mejía</LastName><ForeName>Nasmille L</ForeName><Initials>NL</Initials><AffiliationInfo><Affiliation>School of Environmental Sciences, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gibson</LastName><ForeName>Lisa</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>School of Environmental Sciences, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Farhan Ul Haque</LastName><ForeName>Muhammad</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>School of Environmental Sciences, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ramiro-García</LastName><ForeName>Javier</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Luxembourg Centre for Systems Biomedicine, University of Luxembourg, Esch-sur-Alzette, Luxembourg.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>McGenity</LastName><ForeName>Terry J</ForeName><Initials>TJ</Initials><AffiliationInfo><Affiliation>School of Biological Sciences, University of Essex, Colchester, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Murrell</LastName><ForeName>J Colin</ForeName><Initials>JC</Initials><AffiliationInfo><Affiliation>School of Environmental Sciences, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK. j.c.murrell@uea.ac.uk.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>12</Month><Day>07</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Microbiome</MedlineTA><NlmUniqueID>101615147</NlmUniqueID><ISSNLinking>2049-2618</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001426">Bacterial Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002070">Butadienes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017931">DNA Primers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D045782">Hemiterpenes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0A62964IBU</RegistryNumber><NameOfSubstance UI="C005059">isoprene</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.-</RegistryNumber><NameOfSubstance UI="D006899">Mixed Function Oxygenases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001419" MajorTopicYN="N">Bacteria</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="Y">classification</QualifierName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001426" MajorTopicYN="N">Bacterial Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001673" MajorTopicYN="N">Biodegradation, Environmental</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002070" MajorTopicYN="N">Butadienes</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D042621" MajorTopicYN="N">Comamonadaceae</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017931" MajorTopicYN="N">DNA Primers</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045782" MajorTopicYN="N">Hemiterpenes</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006899" MajorTopicYN="N">Mixed Function Oxygenases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012240" MajorTopicYN="N">Rhodococcus</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012988" MajorTopicYN="N">Soil Microbiology</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D042301" MajorTopicYN="N">Sphingomonadaceae</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Climate</Keyword><Keyword MajorTopicYN="Y">Gene probes</Keyword><Keyword MajorTopicYN="Y">Isoprene</Keyword><Keyword MajorTopicYN="Y">Isoprene monooxygenase</Keyword><Keyword MajorTopicYN="Y">isoA</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>09</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>11</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>12</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>12</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>4</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30526688</ArticleId><ArticleId IdType="doi">10.1186/s40168-018-0607-0</ArticleId><ArticleId 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10;49(6-7):526-31</Citation><ArticleIdList><ArticleId IdType="pubmed">22142727</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Luxembourg (pays)</li><li>Royaume-Uni</li></country><settlement><li>Luxembourg</li></settlement><orgName><li>Université du Luxembourg</li></orgName></list><tree><country name="Royaume-Uni"><noRegion><name sortKey="Carri N, Ornella" sort="Carri N, Ornella" uniqKey="Carri N O" first="Ornella" last="Carri N">Ornella Carri N</name></noRegion><name sortKey="Farhan Ul Haque, Muhammad" sort="Farhan Ul Haque, Muhammad" uniqKey="Farhan Ul Haque M" first="Muhammad" last="Farhan Ul Haque">Muhammad Farhan Ul Haque</name><name sortKey="Gibson, Lisa" sort="Gibson, Lisa" uniqKey="Gibson L" first="Lisa" last="Gibson">Lisa Gibson</name><name sortKey="Larke Mejia, Nasmille L" sort="Larke Mejia, Nasmille L" uniqKey="Larke Mejia N" first="Nasmille L" last="Larke-Mejía">Nasmille L. 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